Master Higgins' girlfriend Tina is kidnapped once again, this time by a group of aliens that have also kidnapped his animal friends. Higgins must once again travel to eight different islands and go after the aliens keeping Tina hostage. The rules of the game are not very different from those of the previous game, Adventure Island II. This time, Higgins can now crouch, an ability he did not have in the previous NES games. However, he loses the ability to backtrack during a stage. Unlike the previous game, the boss does not move to a different stage when the player loses a life.